Content Determination of Triptolide in Tripterygium Wilfordii Cut Crude Drugs by HPLC
- VernacularTitle:高效液相色谱法测定雷公藤饮片中雷公藤内酯醇的含量
- Author:
Xiaoming CHEN
- Publication Type:Journal Article
- Keywords:
HPLC;
Tripterygium wilfordii cut crude drugs;
Wilforlide alcohol;
Content determination
- From:
China Pharmacy
2001;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To develop a RP-HPLC method for the determination of triptolide in tripterygium wilfordii cut crude drugs.METHODS:Samples were extracted with chloroform and acetic ether by ultrasound and underwent neutral alu-mina column elution and isolation,the content determination was performed with acetonitrile-water(23∶77)as mobile phase at a flow rate of1.0ml/min and detection wavelength at218nm.RESULTS:The linear detection concentration range of trip-tolide alcohol was2.74～96?g/ml(r=0.9999),and the average recovery were94.78%(RSD=1.57%)and98.2%(RSD=1.7%),respectively.CONCLUSION:Both methods are applicable for the quality control of triptolide alcohol,yet the separation and determination outcome were better for the sample treated with acetic ether.
