Effect of early inflammation on the efficacy of gene transfer to vein graft
- VernacularTitle:早期炎性反应对移植静脉中外源基因表达效率的影响
- Author:
Tao LUO
;
Qiang ZHANG
;
Jian ZHANG
;
Jianxin LI
;
Yongquan GU
;
Hengxi YU
;
Zhonggao WANG
- Publication Type:Journal Article
- Keywords:
Vein/transpl;
Inflammation Affect;
Gene Expression
- From:
Chinese Journal of General Surgery
1994;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of early inflamation on the efficacy of gene(LacZ gene)(transfer) to vein graft by adenovirus vector.Methods The liquid containing adenovirus vector was infused through external carotid vein and the segment of carotid vein was resected.The veins were inflated naturally.After 30min incubation,the veins were transplanted into carotid artery in experimental group,and the(circulation) was restored in control group.The veins with transferred gene were collected on day 3,day 14 and day 21.The mark gene expression in the veins was studied with X-Gal stain and activities of ?-galactase were measured.ICAM-1 and VCAM-1 expressions were observed by immunohistochemistry stain in transgenic veins.Results The activities of ?-galactase in mark gene expressed for 14 days was decreased and in 21 day nearly disappearred.VCAM-1 and ICAM-1 expression upregulated after day 3 in transplanted veins and there was leucocyte infiltration and shedding of endothelial cells with endothelial disruption.On the other hand,there was no expression of VCAM-1 and ICAM-1 in control group veins and integrity of endothelium was intact.The positive ECs expressed mark gene was obviously reduced in transplanted veins compared to control group.But the number of positive SMCs were not significently different between to two groups.(Conclusions) The inflammation of vein grafts in early stage of transplantation may be the cause of(endotheliocyte) damage and is related to rapid depression of gene expression.
