Status of the proliferation and apoptosis of colorectal mucosa during the mice colon carcinogenesis induced by dimethylhydrazine
- VernacularTitle:二甲基肼诱发小鼠结直肠癌形成过程中增殖与凋亡的研究
- Author:
Chengxia LIU
;
Shangzhong ZHANG
;
Xiaowei ZHANG
;
Tiejun LI
;
Lihua HUANG
;
Jing ZHANG
;
Bing WANG
- Publication Type:Journal Article
- Keywords:
Colorectal Neoplasms/chem index;
Dimelhylhy Drazine/adv eff;
Apoptosis;
Gene Expression
- From:
Chinese Journal of General Surgery
1994;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of imbalance of proliferation and apoptosis in the development of colorectal carcinoma(CRC),and the molecular mechanism of the dynamic change.Methods ORC was(induced) with dimethylhydrazine(DMH) in male mice of Kimming strain.The mice were killed in batches in the 12th,18th and 24th weeks of carcinoma induction.The distribution and extent of proliferation and(apoptosis) of the colorectal mucosa,at various intervals,were dynamically observed.Three genes,p21waf1,Bax and Gadd45 were analyzed by RT-PCR,immunohistochemistry and Western blot.Results During the course of carcinoma induction,the mucosas of the model mice showed sequential changes of atypital(hyperplasia),adenoma,and carcinoma.Compared with control group,the PCNA expression of the model group mice was significantly higher(P
