The appearance of a novel CMY type class C cephalosporinase (AmpC) in southern China
- VernacularTitle:华南地区出现新型CMY型AmpC?-内酰胺酶
- Author:
Weijiao LIAO
;
Jiehua JIANG
;
Jianyun YI
;
Yunjian XU
;
Sizeng PAN
- Publication Type:Journal Article
- Keywords:
Citrobacter freundi;
Beta-Lactamases;
Drug resistance,multiple
- From:
Journal of Chinese Physician
2001;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the antibiotic resistance and molecular epidemiology character of ?-lactamase producers among clinical isolates of Citrobacter freundii.Methods Four strains of Citrobacter freundii were detected by K-B susceptibility method and three-dimensional test.The ?-lactamase gene was identified by polymerase chain reaction(PCR) and sequencing.Results The No.29 strain of Citrobacter friundii was multiple-drug-resistant and proved to produce CMY,DHA type AmpC,TEM and SHV type of extended-spectrum ?-lactamases(ESBLs).The sequencing of corresponding DNA revealed 97% identities of the CMY-2 amino acid sequence.It was a new type of cephalosporinase.Conclusion A novel strain of Citrobacter friundii with CMY type AmpC,DHA type AmpC,TEM and SHV type ESBLs has been found in southern China and it is multiple-drug-resistant.
