The Role of Endothelial Nitric Oxide Synthase in Endothelial Cells Apoptosis Induced by TNF-? and Angiotensin II
- VernacularTitle:内皮型一氧化氮合酶在TNF-?和AngⅡ致内皮细胞凋亡中的作用
- Author:
Ruiwei GUO
;
Lixia YANG
;
Chuanming GUO
;
Feng QI
;
Yankun SHI
- Publication Type:Journal Article
- Keywords:
eNOS;
TNF-?;
AngⅡ;
NF-?B;
Endothelial cells
- From:
Journal of Kunming Medical University
1990;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the role of eNOS and NF-?B in the cultured endothelial cells apoptosis induced by TNF-? and Ang II.Methods The cultured endothelial cells were treated with TNF-? and Ang II in absence and presence of PDTC(an inhibitor of NF-?B);the mRNA of eNOS was measured by RT-PCR,the protein of eNOS and I?B? were assessed by Western-blot,the activity of NF-?B was evaluated by EMSA,and the apoptosis of cells was detected with Tunel.Results The mRNA and protein of eNOS were significantly decreased in the endothelial cells induced by TNF-? and AngII(P
