Preparation of Tretinoin Liposome by Calcium Acetate Gradient Method
- VernacularTitle:醋酸钙梯度法制备维A酸脂质体
- Author:
Jiayi ZHENG
;
Hua HUANG
- Publication Type:Journal Article
- Keywords:
Calcium acetate gradient;
Tretinoin liposome;
Entrapped efficiency;
Quality evaluation
- From:
China Pharmacy
2001;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To evaluate the feasibility of preparing tretinoin liposome by calcium acetate gradient method and to compare this method with the microfilm and the antiphase evaporation method.METHODS:Tretinoin liposome was pre-pared by microfilm method,antiphase evaporation method and calcium acetate gradient method,respectively.Sephadex G-50was used for isolation and ultraviolet spectrophotometry was utilized to determine the entrapped efficiency and grain size of liposomal tretinoin.Electronmicroscope was applied to observe the shape of tretinoin liposome.RESULTS:The calcium ac-etate gradient technology achieved the highest entrapped efficiency of90.48%.The liposomes had perfect shape with the mean diameter of35.8nm and kept stable in quality at4℃for3months.CONCLUSION:The liposome with high entrapped effi-ciency could be prepared by calcium acetate gradient method,and the method is feasible.
