Determination of Kanamycin Sulfate Eye Drops by Dye Colorimetry
- VernacularTitle:染料比色法测定硫酸卡那霉素滴眼液中主药的含量
- Author:
Fulan WANG
;
Zhen WU
- Publication Type:Journal Article
- Keywords:
Dye colorimetry;
Kanamycin sulfate eye drops;
Bromothymol green;
Content determination
- From:
China Pharmacy
2001;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for content determination of principal agents in kanamycin sulfate eye drops by dye colorimetry.METHODS:On the basis of the spectrometric features of ion pair compound produced in the reaction between kanamycin and bromcresol green in buffer solution with pH value of6.0,absorbance was detected at the wavelength of432nm and content was determined.RESULTS:The detected concentration of Kanamycin showed good linear relationship with its absorbance(r=0.9998)within the range of18.0～72.0?g/ml.The average recovery was99.3%(RSD=1.20%).CONCL_ USION:The established method is rapid,simple and accurate,and it can be used for quick test of this preparation during the production.
