Detection of disseminated tumor cells in the peripheral blood of patients with laryngeal or laryngopharyngeal carcinomas
- VernacularTitle:喉、喉咽癌患者外周血播散肿瘤细胞检测的初步研究
- Author:
Pingdong LI
;
Zhenkun YU
;
Zhigang HUANG
;
Jugao FANG
;
Xin NI
;
Qi WANG
;
Erzhong FAN
;
Ying LI
- Publication Type:Journal Article
- Keywords:
Laryngeal Neoplasms;
Hypopharyngeal Neoplasms;
Neoplasms Metastasis;
Neoplasms Circulating Cells;
Revorse sTranscriptase Polymerase Chain Reaction
- From:
Chinese Archives of Otolaryngology-Head and Neck Surgery
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the diagnostic and prognostic value of molecular biological detection of DTC in peripheral blood. METHODS 32 cases of laryngeal or laryngopharyngeal carcinoma were investigated. DTC in peripheral blood was detected by nested reverse transcription polymerase chain reaction,using CK19mRNA as the marker. RESULTS In the RT-PCR study,15 of 32 cases (46.9 %) showed a positive result. Ten of the 25 cases (40 %) of laryngeal carcinoma were positive. Fix of the remaining 7 cases (71.4 %) of laryngopharyngeal carcinoma were positive. All controls were negative. Of the 20 cases without lymph node metastasis,6 were positive; of the 12 cases with lymph node metastasis,9 were positive. The positive rate of the group with lymph node metastasis was higher than that of the group without lymph node involvement(P
