Cloning of the pneumolysin gene
- VernacularTitle:肺炎球菌溶血素基因的克隆
- Author:
Bing CHEN
;
Wenjia DAI
;
Zhengmin WANG
;
Zhongming LI
- Publication Type:Journal Article
- Keywords:
Vaccines;
Otitis Media;
Streptococcus pneumoniae;
Hemolysins
- From:
Chinese Archives of Otolaryngology-Head and Neck Surgery
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To prepare pneumolysin(Pn)by genetic engineering and thereby establish the basis for the study of vaccines against otitis media. METHODS A pair of primers including two restriction sites was designed based on the pneumolysin gene sequence reported by Walker in 1987. The pneumolysin gene was PCR-amplified from pneumococcal DNA. The resulting fragment, digested by restriction enzymes, was ligated into the vector PET-28a and then transformed into host cell E.coli JM109(DE3). RESULTS The sequence of the inserted pneumolysin gene was confirmed by DNA sequencing. CONCLUSION The pneumolysin gene was successfully cloned into the host cell.
