The differential expression of somatostastin receptors in hepatocellular carcinoma HCCLM3 cell lines
- VernacularTitle:人肝癌细胞系HCCLM3中生长抑素受体亚型的表达研究
- Author:
Zhaohui ZHONG
;
Shu LI
;
Jirun PENG
;
Jiye ZHU
;
Xisheng LENG
;
Mai ZHOU
;
Gangjun JIAO
;
Yantong GUO
;
Xun HUANG
;
Yuhua WEI
;
Xin YU
- Publication Type:Journal Article
- Keywords:
Carcinoma, hepatocellular;
Receptor, somatostatin;
mRNA
- From:
Chinese Journal of General Surgery
1997;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mRNA and protein expression of somatostatin receptors in hepatocellular carcinoma HCCLM3 cell lines and to explore the mechanism by which somatostatin effects on hepatocellular carcinoma. Methods RT-PCR., immunocytochemistry and MTT were used to detect mRNA and protein expression of somatostatin receptors in hepatocellular carcinoma cells and evaluate the antiproliferative effect of somatostatin. Results The effect of somatostatin on the cellular proliferation was verified. Immunocytochemistry study revealed a mainly intracellular distribution of all SSTRs with unique patterns for each of them. mRNA expression of all 5 subtypes of somatostatin receptors was different, SSTR2 and SSTR1 mRNA expressions were significantly higher than SSTR3, SSTR4 and SSTR5 ( P
