Effects of midazolam, ketamine and propofol on cognitive function in aged rats
- VernacularTitle:咪唑安定、异丙酚和氯胺酮对老年大鼠认知功能的影响
- Author:
Yuhe LIU
;
Xinmin WU
;
Minyi DU
- Publication Type:Journal Article
- Keywords:
Midazolam;
Propofol;
Ketamine;
Cognition disorders;
Receptors, N-methyl-D-aspartate
- From:
Chinese Journal of Anesthesiology
1994;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of midazolam, ketamine and propofol on spatial cognitive function and the mRNA expression of N-methyl-D-aspartate (NMDA) receptor subunits NR1 and NR2B in the hippocampus of aged rats. Methods Thirty-two Wistar rats of both sexes aged 18 months were randomly divided into 4 groups: Ⅰ control group (n = 9); Ⅱ midazolam group (M, n = 8);Ⅲ propofol group (P, n = 7) and Ⅳ ketamine group (K, n = 8). The animals received intraperitoneal (IP) midazolam 30 mg?kg-1 or propofol 60 mg ?kg-1 or ketamine 80 mg?kg-1 once a day for 3 days whereas the animals in control group received IP normal saline 2 ml instead. One day after the last drug administration the animals underwent Morris water maze test 4 times a day for 3 consecutive days. The animals were killed at 1h after last test and the brains were immediately removed for determination of NR1 and NR2B mRNA expression in the hippocampus using RT-PCR.Results The latency period and swimming distance were significantly shorter on the 3rd day of water maze test than on the 1st day in control group and group K, P. The latency period on the 1st day was significantly longer in group K than in control group. The NR1 mRNA expression in group M was significantly higher while the NR2B mRNA expression in group K and P was significantly lower than that in control group (P
