Effects of Matrine in Combination with 3 Antitumor Drug on Cell Cycle of KBV200 Cell Line
- VernacularTitle:苦参碱与3种抗肿瘤药物联合作用对KBV200耐药细胞株细胞周期的影响
- Author:
Hongyan CHEN
;
Yan CHEN
;
Caifeng LV
;
Xiaoyan YU
;
Na SHEN
;
Qianying ZHANG
;
Ling YE
- Publication Type:Journal Article
- Keywords:
Matrine;
Antitumor drug;
KBV200 cell line;
Cell cycle
- From:
China Pharmacy
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To observe the effects of matrine in conjunction with vincristine(VCR),adriamycin(ADM)and diamminedichloroplatinum(DDP),respectively on the cell cycle of the KBV200drug-resistant cell line.METHODS:The cultured KBV drug-resistant cells were divided into control group,antitumor drug groups and the combination therapy groups(marine+antitumor drugs,respectively),FCM assay was used to detect and analyze the expression of cell cycle and apoptosis of each group.RESULTS:As compared with single antitumor drugs groups,no significant change was found in the proportion of cells and cell apoptosis in the combination therapy groups(matrine+VCR or matrine+ADM).But marine+DDD resulted in a reduction of the G 0 ～G 1 stage cell population but an increase of G 2 ～M stage cell population.CONCLUSIONS:It was assumed that matrine-induced reversal of KBV200cells'drug-resistant to VCR and ADM may not relate to cell cycle and cell apoptosis.Matrine can slightly enhance the cytotoxic effect of DDP.
