Triptolide induces apoptosis of rat synoviocytes in collagen-induced arthritis
- VernacularTitle:雷公藤甲素诱导胶原导致的关节炎大鼠滑膜细胞凋亡的研究(英文)
- Author:
Shenghao TU
;
Yonghong HU
;
Gengmin WENG
;
Fuer LU
;
Mingmin ZHANG
;
Xianyang LAI
;
Peilin LIU
- Publication Type:Journal Article
- Keywords:
Triptolide;
Synovial membrane;
Apoptosis;
Arthritis, experimental
- From:
Chinese Journal of Pathophysiology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To determine whether triptolide induce apoptosis of synovial cells in collagen-induced arthritis (CIA) in rats. METHODS: The male Wistar rats were used to make CIA models by immunized with Bovine collagen Ⅱ (BCⅡ) in Freund's complete adjuvant (FCA). A total of 20 CIA rats were randomly divided into 2 groups, triptolide group (10 rats) and CIA control group (10 rats). Triptolide group were administered with triptolide at 40 ?g/kg body weight intramuscularly every three days. CIA control group and another 10 age-matched normal rats were given normal saline instead. The rats were sacrificed on the 31st day after the triptolide administration. The pieces of synovium of the rat knee joints were harvested. The synovium was examined by HE staining and electron microscope. The apoptosis was tested by TUNEL and flow cytometer. RESULTS: The earlier phase of apoptotic synoviocytes were observed under the electron microscope. The flow cytometry showed that the percentage of the apoptotic cells was (3.98?1.16)% in the triptolide group, (1.83?0.82)% in the CIA control group, and (0.87?0.24)% in the normal group (P
