Expression of MHC Ⅱ on rat corneal keratocytes is inhibited by special siRNA targeting CⅡTA
- VernacularTitle:靶向CⅡTA基因siRNA对大鼠角膜基质细胞MHCⅡ基因表达影响的研究
- Author:
Xuefei ZHANG
;
Jiaqi CHEN
;
Yingfeng SHAO
- Publication Type:Journal Article
- Keywords:
Genes, CⅡTA;
Genes, MHCⅡ;
siRNA;
Cornea
- From:
Chinese Journal of Pathophysiology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the feasibility to inhibit the expression of MHCⅡ by special siRNA targeting class Ⅱ major histocompatibility complex (MHC Ⅱ) transactivator (CⅡTA), which might regulate MHC Ⅱexpression for suppressing immune rejection. METHODS: Five different siRNA were designed, synthesized and transfected into freshly isolated rat corneal keratocytes. At 24 hours posttransfection, the changes of MHC Ⅱexpression were detected by flowcytometry, and the mRNA abundance of CⅡTA and MHC Ⅱ was measured by FQ-PCR after inducing with recombinant rat interferon-gamma (IFN-?). RESULTS: Different siRNA showed different reduction in MHC Ⅱ and CⅡTA expression compared with the control (P
