Aldehyde dehydrogenase 2 prevents rat cardiomyocytes from apoptosis induced by hypoxia
- VernacularTitle:乙醛脱氢酶2在大鼠心肌缺氧损伤中的抗凋亡作用
- Author:
Danling XU
;
Aijun SUN
;
Shijun WANG
;
Han FU
;
Jianguo JIA
;
Keqiang WANG
;
Yunzeng ZOU
;
Junbo GE
- Publication Type:Journal Article
- Keywords:
Aldehyde dehydrogenase;
Hypoxia;
Myocardium;
Apoptosis
- From:
Chinese Journal of Pathophysiology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To observe the effect of hypoxia on cardiaomyocytes apoptosis and the role of ALDH2 in the process. METHODS: Cultured cardiomyocytes of neonatal rats were used. Hypoxia was imposed to the cardiomyocytes with or without daidzin pretreatment. ALDH2 activity was measured by the method of acetaldehyde metabolism. Apoptosis was measured by Hoechest 33324 staining, fluorescence activated cell sorting (FACS) and the DeadEnd~ TM fluorometric TUNEL system. RESULTS: ALHD2 enzyme activity in myocytes was inhibited by daidzin (24 h, 60 ?mol/L) without induction of apoptosis. When exposed to hypoxia, however, the apoptisis was significantly increased in the cells pretreated with daidzin compared to those without the pretreatment. CONCLUSION: The reduction of ALDH2 activity might increase the susceptivity of myocytes to apoptosis following hypoxia, suggesting a protective role of ALDH2 in hypoxia-induced myocardial injury.
