Inhibitory effect of ferulate on attachment and migration induced by fibronectin and fibrinogen in cultured vascular smooth muscle cells from SHR
- VernacularTitle:阿魏酸钠抑制纤维粘连蛋白和纤维蛋白原诱导的大鼠血管平滑肌细胞迁移和粘附
- Author:
Ying HAN
;
Liangdi XIE
;
Changsheng XU
;
Huajun WANG
- Publication Type:Journal Article
- Keywords:
Ferula;
Fibrinogen;
Fibronectins;
Cell movement;
Muscle, smooth, vascular
- From:
Chinese Journal of Pathophysiology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effect of sodium ferulate (SF), one of the principal components of rhizoma ligustici wallichii, on the attachment and migration induced by fibronectin (FN) and fibrinogen (Fg) in vascular smooth muscle cells (VSMCs). METHODS: Cultured VSMCs derived from spontaneously hypertensive rats (SHR) were used. Cell attachment was conducted using a flat-bottomed 96 well polystyrene plate. Cell migration was determined by modified Boyden chamber assays. Intracellular free calcium (Ca~ 2+ i) was measured with fluorescent Ca~ 2+ indicator Fura-2/AM. RESULTS: FN and Fg significantly induced the attachment and migration of VSMCs in a dose-and time-dependent manner, which was inhibited by pre-treatment of VSMCs with SF (10~ -7 -10~ -3 mol/L) a dose-dependentl fashion. The peak inhibition rate of attachment induced by FN and Fg was 67.12% and 70.23%, respectively, and the peak inhibition rate of SF on migration induced by FN and Fg was 69.79% and 87.06% (P
