Changes of NO- and H_2O_2-dependent soluble guanylate cyclase pathway in the hypoxic hypercapnic pulmonary hypertension rats
- VernacularTitle:肺动脉高压大鼠一氧化氮及过氧化氢依赖的可溶性鸟苷酸环化酶通路的变化
- Author:
Xiaodong XIA
;
Lei YANG
;
Zhengjie XU
;
Yuanrong DAI
;
Shuzhen WU
;
Hongqin ZHANG
- Publication Type:Journal Article
- Keywords:
Anoxia;
Hypercapnia;
Pulmonary hypertension;
Guanylate cyclase;
Free radicals;
Nitric oxide;
Signal transduction
- From:
Chinese Journal of Pathophysiology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the effect of hypoxia and hypercapnia on nitric oxide (NO) in plasma and superoxide dismutase (SOD), catalase (CAT), soluble guanylate cyclase (sGC), cyclic guanosine monophospholate (cGMP) in lung tissue in rats, and to explore the effect of NO- and H_2O_2-sGC pathway on the development of the pulmonary hypertension. METHODS: The model of hypoxic and hypercapnic 1, 2, 4-week group (HH 1 week, HH 2 weeks, HH 4 weeks) and control group was set up. NO content in plasma, CAT and SOD in rat lung were determined by spectrophotometry. The sGC activity in lung tissue was detected by enzyme kinetic analysis. cGMP content in lung tissue was examined with ~ 125 I-radioimmunoassay. RESULTS: The mean pulmonary artery pressure (mPAP) showed significantly higher in HH 1 week, HH 2 weeks and HH 4 weeks groups compared with control group (all P
