Effects of Shenmai injection on I?B and TLR4 mRNA expression in lungs of rats exposed to hemorrhagic and LPS shock
- VernacularTitle:“失血加LPS”大鼠肺脏I?B?和TLR4基因表达及参麦的肺脏保护作用
- Author:
Zhaoxin YUAN
;
Xiao LIU
;
Dongping JI
;
Xu ZHANG
;
Zhi WANG
;
Yang LI
;
Xuejian ZHAO
;
Liankun SUN
- Publication Type:Journal Article
- Keywords:
Drugs, Chinese herbal;
Dexamethasone;
Lipopolysaccharides;
Receptor, Toll-like;
I?B?;
Tumor necrosis factor
- From:
Chinese Journal of Pathophysiology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To explore the molecular mechanism of anti-shock effect of Shenmai (SM) injection and to observe its influence on TLR4 and I?B mRNA expression in hemorrhagic shock (HS) plus LPS rat lung. METHODS: Rats were divided into HS, HS+LPS, HS+LPS+ dexamethasone (DEX), HS+LPS+SM group, respectively. HS+LPS injury model of rats were induced by hemorrhagic shock (MAP 40 mmHg for 10 min) followed by intravenous administration of lipopolysaccharide (LPS, 1.5 mg/kg). Four hours later, the TNF-? contents in lung tissue were measured by ELISA, TLR4 mRNA and I?B? mRNA expression were assayed by RT-PCR, ultrastructural changes of the type Ⅱ alveolar cell were observed by electron microscope. RESULTS: The TLR4 mRNA expression level in HS+LPS+SM group was significantly decreased as compared to HS+LPS group (P
