Chrysanthemum morifolium Ramat sensitizes TNF-related apoptosis inducing ligand-induced apoptosis in human colon cancer cell line DLD-1
- VernacularTitle:杭白菊增强TRAIL基因诱导大肠癌细胞凋亡及机制的实验研究
- Author:
Wenxian HU
;
Weifang MAO
;
Chao HE
;
Lei ZHANG
;
Xuefeng HUANG
;
Weidong DU
;
Bingliang FANG
- Publication Type:Journal Article
- Keywords:
Chrysanthemum morifolium Ramat;
Tumor necrosis factor-related apoptosis-inducing ligand;
Colorectal neoplasms;
Apoptosis
- From:
Chinese Journal of Pathophysiology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To explore the influence of Chrysanthemum morifolium Ramat on TNF-related apoptosis inducing ligand (TRAIL)-mediated apoptosis in human colon cancer cell line DLD-1 and its possible mechanism. METHODS: Adenovirus-mediated TRAIL gene AD/hTERT-gTRAIL was applied either alone or by combination with Chrysanthemum morifolium Ramat in human colon DLD-1 cell line. Cell growth and apoptosis were measured by inverted microscope, MTT method and flow cytometry. The expression of TRAIL mRNA, TRAIL-Rs mRNA and TRAIL protein expression after exposure to Chrysanthemum morifolium Ramat were measured by semi-quantitive RT-PCR and FACS, respectively. RESULTS: The suppression percentages and apoptotic rate of DLD-1 by Ad/hTERT-gTRAIL alone were 31.4% and 13.5%, respectively. Combination of TRAIL gene transfection with Chrysanthemum morifolium Ramat, the suppression and the apoptosis rate raised to 93.1% and 45.4%, respectively (P
