Dynamic changes of glucosylceramide synthase and caspase 3 in drug resistance induced by doxorubicin in human gallbladder carcinoma cell line GBC-SD
- VernacularTitle:阿霉素诱导人胆囊癌细胞耐药过程中糖基化神经酰胺合成酶及caspase 3的动态变化
- Author:
Jiangtao LI
;
Shuyou PENG
;
Xinbao WANG
;
Yingbin LIU
;
Jianwei WANG
;
Bin XU
;
Haijun LI
;
Xuedong FENG
;
Haoran QIAN
;
Haijun WANG
;
Yulian WU
;
Heqing FANG
- Publication Type:Journal Article
- Keywords:
Gallbladder neoplasms;
Drug resistance;
Ceramides;
Caspase 3;
Doxorubicin
- From:
Chinese Journal of Pathophysiology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the levels of mRNA, protein of glucosylceramide synthase (GCS) and caspase 3 in the drug resistance induced by doxorubicin in human gallbladder carcinoma cell line GBC-SD, the effect of ceramide metabolism in this process was examined. METHODS: Human gallbladder carcinoma cell line GBC-SD was treated by doxorubicin at concentration of 200 ?g/L for 12 weeks (named GBC-SD12). Cytotoxicity, mRNA and protein of GCS were measured on 1st week, 4th week and 12th week by MTT assays, RT-PCR or Western blotting. The levels of caspase 3 were measured by spectrofluorometry. RESULTS: A 3.8-fold increase in drug resistance to doxorubicin in GBC-SD12 was observed. Up-regulation of GCS mRNA and protein were also detected in GBC-SD12 (P
