Inhibition of telomerase activity in colon cancer LS-174T cells with liposome-mediated cripto antisense oligodeoxynucleotide
- VernacularTitle:脂质体介导的cripto反义寡核苷酸抑制结肠癌细胞端粒酶活性
- Author:
Yu FAN
;
Shu ZHENG
;
Jiayi DING
- Publication Type:Journal Article
- Keywords:
Oligonucleotides, antisense;
Telomerase;
Genes, cripto;
Colonic neoplasms
- From:
Chinese Journal of Pathophysiology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the effects of cripto antisense oligodeoxynucleotide (ASODN) on telomerase activity in colon cancer LS-174T cells. METHODS: Colon cancer LS-174T cells were treated with cripto ASODN. The expression of cripto mRNA was studied using real-time PCR. The telomerase activity was detected by telomeric repeat amplification protocol enzyme-linked immunoabsordent assay (TRAP-ELISA). The growth of LS-174T cells was observed with soft agar. RESULTS: Cripto ASODN inhibited the growth of LS-174T cells in a dose-dependent manner in soft agar. Cripto ASODN decreased the telomerase activity in colon cancer LS-174T cells, and the effects of repression were time-and dose-dependent. CONCLUSION: Cripto may contribute to the activation of telomerase in colon cancer.
