Effects of lovastatin on differentially expressed genes in HepG2 cells
- VernacularTitle:Lovastatin对肝癌HepG2细胞作用后基因差异表达的初步研究
- Author:
Baoan WU
;
Lijian LIANG
;
Shaoqiang LI
;
Xiaojun LI
;
Baogang PENG
- Publication Type:Journal Article
- Keywords:
HepG2 cells;
Lovastatin;
Gene expression
- From:
Chinese Journal of Pathophysiology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To analyze the lovastatin-induced differential gene expression in HepG2 cells using a cDNA microarray assay. METHODS: Total RNA was extracted from the lovastatin-treated HepG2 cells and control group. cDNA was synthesized from RNA with Cy3/Cy5-labelled dCTP. Then the hybridization was conducted. The result was analyzed using Imagene and Genespring software. RT-PCR was carried to confirm the hybridization results. RESULTS: 30 genes were up-regulated while 11 genes were down-regulated in lovastatin-treated HepG2 cells, involved in some major functional areas including signal transduction, cell cycle regulation, tumor immunity, and so on. CONCLUSION: The analysis of differentially expressed genes in lovastatin-treated HepG2 cells is helpful to explore the mechanism of the anti-tumor activity of statins.
