Differentiation of mesenchymal cells derived from mouse fetal liver into skeletal muscle-like cells in vitro
- VernacularTitle:小鼠胎肝间质干细胞体外向肌样细胞分化的研究
- Author:
Hua JIANG
;
Yuan ZHANG
- Publication Type:Journal Article
- Keywords:
Fetal liver;
Mesenchymal stem cells;
Skeletal muscle cells;
Cell differentiation
- From:
Chinese Journal of Pathophysiology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study whether mesenchymal stem cells (MSCs) from fetal liver can differentiate into skeletal muscle-like cells. METHODS: MSCs were isolated from C57BL/6J mouse fetal liver and were induced by 5-azacytidine and amphotericin B. Myf5 and myogenin were tested by RT-PCR. Desmin and ?-actin was examined by immunocytochemistry. RESULTS: RT-PCR showed that the treated cells expressed Myf5 and myogenin orderly from 6 hours to 72 hours, while the untreated cells did not. Immunocytochemistry confirmed that these cells were positive for desmin and ?-actin and the positive rate was higher in 7 days than that in 14 days. CONCLUSION: MSCs derived from fetal liver can be induced into skeletal muscle-like cells in vitro.
