Effect of siRNA targeting survivin gene on the apoptosis of malignant melanoma cell line A375
- VernacularTitle:siRNA抑制生存素基因对恶性黑素瘤细胞A375凋亡的影响
- Author:
Siyuan CHEN
;
Jing CHENG
;
Changzheng HUANG
;
Yue QIAN
;
Yating TU
- Publication Type:Journal Article
- Keywords:
Melanoma;
RNA, small interfering;
Apoptosis;
Survivin
- From:
Chinese Journal of Dermatology
1995;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of siRNA targeting survivin gene on the apoptosis of malignant melanoma cell line A375. Methods The eucaryotic expression vector of pU-survivin-siRNA was constructed and transfected into the A375 cells by electroporation. The protein expression of survivin was examined by Western blotting, and cell apoptosis by flow cytometry. Results The transfection of pU-sur-vivin-siRNA significantly down-regulated survivin expression ( 0.24 ?0.02 in the transfected group versus 0.98 ?0.21 in the control group ) in A375 cells, and promoted cell apoptosis ( 83% in the transfected group versus 28% in the control group, P
