Effects of siRNA targeting CD147 gene on the expression of CD147 and the proliferation of malig- nant melanoma cell line A375
- VernacularTitle:CD147 siRNA对恶性黑素瘤细胞株A375增殖及CD147表达的影响
- Author:
Xiang CHEN
;
Jing LIN
;
Wei LIN
;
Juan SU
;
Mingliang CHEN
;
Wei SHI
;
Hongfu XIE
;
Fuwen CHEN
- Publication Type:Journal Article
- Keywords:
RNA, small interfering;
Melanoma;
Cell proliferation;
CD147
- From:
Chinese Journal of Dermatology
1995;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of siRNA targeting CD 147 gene on the expression of CD147 in melanoma cell line A375, and on proliferation of these cells. Methods Previously prepared recombinant plasmid pSUPER/CD147 siRNA was used. In this study, the recombinant was transfected into the A375 cells. The mRNA expression of CD147 was measured by semi-quantitive RT-PCR, the proliferation of the cells by MTT assay. Results After the transfection with pSUPER/CD147 siRNA1 and pSU-PER/CD147 siRNA2, the mRNA expression of CD147 in the A375 cells was significantly down-regulated by 90.81% (P
