Trichostatin A, a Histone Deacetylase Inhibitor, Potentiated Cytotoxic Effect of Ionizing Radiation in Human Head and Neck Cancer Cell Lines.
- Author:
Jin Ho KIM
1
;
Jin Hee SHIN
;
Eui Kyu CHIE
;
Hong Gyun WU
;
Jae Sung KIM
;
Il Han KIM
;
Sung Whan HA
;
Charn Il PARK
;
Wee Saing KANG
Author Information
1. Department of Radiation Oncology, Seoul National University College of Medicine, Seoul, Korea. ihkim@snu.ac.kr
- Publication Type:Original Article
- Keywords:
Trichostatin A;
Histone deacetylase inhibitor;
Radiosensitization;
Head and neck cancer
- MeSH:
Cell Line*;
Cell Survival;
Glioblastoma;
Head and Neck Neoplasms*;
Head*;
Histone Deacetylase Inhibitors*;
Histone Deacetylases*;
Histones*;
Humans*;
Radiation, Ionizing*;
Radiation-Sensitizing Agents
- From:The Journal of the Korean Society for Therapeutic Radiology and Oncology
2004;22(2):138-144
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: We have previously reported that human glioblastoma cells are sensitized to radiation-induced death after their exposure to trichostatin A (TSA), a histone deacetylase inhibitor (HDAC-I), prior to the irradiation. We aimed to measure the magnitude of the radiosensitizing effect of TSA in human head and neck cancer cell lines. MATERIALS AND MEHTODS: Human head and neck cancer cell lines, HN-3 and HN-9, were exposed to 0, 50, 100, and 200 nM TSA for 18 hr prior to irradiation. Then, the TSA-treated cells were irradiated with 0, 2, 4, 6, and 8 Gy, and cell survival was measured by clonogenic assay. RESULTS: Pre-irradiation exposure to TSA was found to radiosensitize HN-3 and HN-9 cell lines. In HN-9 cells, the fraction surviving after 2 Gy (SF2) was significantly reduced by treatment of TSA at concentration as low as 50 nM. However, a treatment with 200 nM TSA was required to significantly decrease SF2 in the HN-3 cell line. SER of pre-irradiation treatment with 200 nM TSA was 1.84 in HN-3 and 7.24 in HN-9, respectively. CONCLUSIONS: Our results clearly showed that human head and neck cancer cell lines can be sensitized to ionizing radiation by pre-irradiation inhibition of histone deacetylase (HDAC) using TSA, and that this potentiation might well be a general phenomenon.
