Experimental study on oligopeptide K16GRGDSPC as a targeted vector for gene transfection
- VernacularTitle:新型靶向性非病毒基因转移载体K16GRGDSPC介导基因转染的实验研究
- Author:
Haitao PAN
;
Qixin ZHENG
;
Xiaodong GUO
;
Yong LIU
;
Changwen LI
;
Yulin SONG
- Publication Type:Journal Article
- Keywords:
Gene transfer techniques;
Myeloid progenitor cells;
Glycine;
Arginine;
Serine;
Aspartic acid;
Lysine;
Proline;
Cysteine
- From:
Journal of Chinese Physician
2001;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the feasibility and efficiency of K16GRGDSPC(K16-RGD) for exogenous gene transfer to bone marrow derived stroma cells(BMSCs).Methods The peptide K16-RGD was synthesized by solid-phase batch peptide synthesizer.The K16-RGD was used as vector for transfecting the luciferase into BMSCs and the expression of the luciferase gene was monitored.The influence of chloroquine and polyethyleneimine was observed.The targeted specificity was examined by cell attachment test and transfection inhibitation test.Results The transfection efficiency of K16-RGD vector was lower than that of commercial lipofectamine.But the efficency of K16-RGD was greatly enhanced in the presence of chloroquine and polyethyleneimine.The peptides containing RGD inhibited the BMSCs attachment to the 96-well plates and decreased the transfection efficiency of K16-RGD significantly.Conclusion Peptide K16GRGDSPC is a new kind of targeted-nonviral gene delivery vector,which is easy to be synthesized,high efficiency and low cytotoxicity.
