In vitro study of tissue engineering cartilage
- VernacularTitle:组织工程化软骨的体外培养
- Author:
Jianhui YUAN
;
Jianyi XIONG
;
Jianjun LIU
;
Daping WANG
- Publication Type:Journal Article
- Keywords:
Myeloid progenitor cells;
Cartilage
- From:
Journal of Chinese Physician
2001;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the culture conditions of bone marrow stromal cells(MSCs) to form the tissue engineering cartilage. Methods The MSCs were obtained by the method of primary and passage culture in vitro.Chondrocytes derived from MSCs were obtained with high initial cell density subculture.The cells were cultured and transferred to the 3rd generation,and mixed with fibrin sealing(FS,5?10~6cells/ml).The conformation and growing feature of the tissue engineering cartilage were observed.Results The cultivated MSCs had shown evident reproductive activity and high rate of adherence.The histological results showed that cartilage matrix was stained positively for toluidine blue.Conclusion Chondrocytes can be derived from MSCs and form into the tissue engineering cartilage with FS.
