Experimental study of optimized bioactive glass by osteoblasts differentiated from rabbit bone marrow mesenchymal stem cells
- VernacularTitle:骨髓间充质干细胞分化为成骨细胞优化生物活性玻璃的实验研究
- Author:
Jinyong DING
;
Anmin JIN
;
Meixia ZHANG
;
Shaoxiong MIN
;
Shanhua TANG
- Publication Type:Journal Article
- Keywords:
Myeloid progenitor cells;
Osteoblasts;
Glass;
Artificial limbs
- From:
Journal of Chinese Physician
2001;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the growth of rabbit osteoblast on the composite bioactive glass material scaffolds and to explore the experimental methods of optimized material scaffolds in bone tissue engineering. Methods The mesenchymal stem cells(MSCs) were separated and cultured from rabbit thigh marrow,and they were induced and differentiated into osteoblast by the revulsant.The change of typical appearance of the MSCs was investigated under microscope.The cytological characteristics of the MSCs were observed through cells activity and immunohistochemistry method.The osteoblast was cocultured with three various bioactive glasses respectively.The compatibility between the various bioactive glasses and osteoblast was compared by observation of the changes of the cells.Results The MSCs were successfully induced and cultured in the presence of the osteoblast revulsant.After inducement,these cells displayed osteoblast-like morphology.The bioactive glass composite scaffolds supported the attachment of cultured rabbit osteoblast.These cells proliferated faster on scaffolds with higher poriness of 90.20% and 94.50% than with lower poriness of 75.90%.Conclusion It is feasible to use bioactive glass composite scaffolds with proper poriness for bone tissue engineering.
