Experimental study of angiogenin factors secreted by bone marrow mononuclear cells cultured in vitro
- VernacularTitle:体外培养骨髓单个核细胞分泌促血管生长因子的实验研究
- Author:
Zhihong LI
;
Xiangsheng ZHANG
;
Xiaoming CHEN
;
Xu ZHANG
- Publication Type:Journal Article
- Keywords:
Vascular endothelial growth factors;
Fibroblast growth factor 2;
Interleukin-1;
Bone marrow cells;
Monocytes
- From:
Journal of Chinese Physician
2001;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To define the secretory capability of angiogenin factors by bone marrow mononuclear cells(BM-MNC) cultured in vitro.Methods Rat BM-MNCs were isolated by centrifugation through a Histopaque density gradient and cultured for four weeks in vitro.The levels of vascular endothelial cell growth factor(VEGF),basic fibroblast growth factor(bFGF) and interleukin-1?(IL-1?) were tested by enzyme-linked immunosorbent assay(ELISA).Results The levels of VEGF in the conditioned medium at 1,2,3 and 4 weeks were(24.40?7.99),(89.28?5.13),(115.24?10.08) and(157.00?15.64) pg/ml,respectively.The levels of bFGF at 1,2,3 and 4 weeks were(52.72?2.13),(48.10?6.41),(44.71?3.21) and(25.61?2.42) pg/ml respectively,and those of IL-1? were(31.28?5.44),(71.87?3.01),(55.77?11.94) and(41.75?9.14),respectively.Conclusion The BM-MNCs can persistently secrete potent angiogenic cytokines such as VEGF,bFGF and IL-1?.
