Establishment and evaluation of indirect competitive enzyme-linked immunosorbent assay for detecting the concentration of trypsiongen activation peptides
- VernacularTitle:尿胰蛋白酶原激活肽间接竞争ELISA检测法的建立及方法学评价
- Author:
Hui WANG
;
Wei LUO
;
Xia XU
- Publication Type:Journal Article
- Keywords:
Trypsinogen;
Peptides;
Enzyme-linked immunosorbent assay
- From:
International Journal of Laboratory Medicine
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an ELISA method for detection of the concentration of TAP in human urine samples.Methods TAP-BSA was used as coating antigen. The TAP was a competitor to TAP-BSA. They reacted to the limited amount of monoclonal antibody against TAP.Results The optimal concentration of the coating antigen was 250ng/ml.The dilutions of monolclonal antibody against TAP and sheep anti-mouse IgG were 25?g/ml and 1∶4 000 respectively. The optimal range was from 0.69 to 1 000ng/ml.The assay provided a sensitivity of 0.69ng/ml.The coefficients of variation of intra-assay and inter-assay were 9.10% and 10.33% respectively. The average recovery rate of TAP was 97.70%.Conclusion The indirect competitive enzyme-linked immunosorbent assay is established.
