Investigation on Drug Resistance of Influenza H3 Subtype Virus Amantadine:A Report from 2003 to 2005 in Bejing District
- VernacularTitle:2003年～2005年北京地区甲3型流感病毒流行株金刚烷胺耐药性调查
- Author:
Hong XU
;
Hang XU
- Publication Type:Journal Article
- Keywords:
Influenza virus;
Drug resistance;
M2 ion channel;
Amantadine
- From:
China Pharmacy
1991;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To provide the reference for the set-up of precautions against flu pandemic in China.METHO_DS:RNA of influenza viruses isolated from flu children in flu seasons between 2003 and 2005 in Beijing was extracted and amplification of M2 gene fragments associated with ion channel were performed by one step reverse transcription–polymerase chain reaction(RT-PCR).The sequencing of amino acid was determined,and its bioinformation was analyzed with software,then the positions of amino acid for resistance to amantadine were detected.RESULTS:20 of 31 isolated strains were resistant to amantadine,the percentage of which was 66.7%.CONCLUSION:The monitoring of drug resistance to flu viruses should be strengthened in China.
