Neuronal stem cells in facial nerve tissue engineering
- VernacularTitle:神经干细胞应用于组织工程化人工神经的实验研究
- Author:
Baofeng GUO
;
Mingmin DONG
;
Xiuhua REN
- Publication Type:Journal Article
- Keywords:
Animals, Laboratory;
Facial Nerve;
Nerve Regeneration;
Nerve Growth Factor;
Tissue Engineering
- From:
Chinese Archives of Otolaryngology-Head and Neck Surgery
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To evaluate the effectiveness of applying neuronal stem cells to bridging a 10mm long facial nerve defect in rabbits using a tissue engineered artificial nerve. METHODS Thirty-six Japanese white rabbits were randomly divided into three groups of 12. Group A: chitosan guidance channels with collagen protein sponge were filled with neuronal stem cells and NGF; Group B: chitosan guidance channels with collagen protein sponge were filled with NGF; Group C: autograft nerve. A series of examinations were performed, including electrophsiological methods, histological staining of nerve, Brdu and S100 immunohistochemical staining, at 12 weeks after operation. RESULTS At 12 weeks after operation, all examination results from Group A were better than from Group B(P0.05). CONCLUSION Neuronal stem cells can be used as seeding cells in peripheral nerve tissue engineering for repairing 10mm facial nerve defects.
