Surveillance of bacterial resistance and study on ?-lactamases in cefoxitin-resistant Klebsiella pneumoniae isolates
- VernacularTitle:耐头孢西丁肺炎克雷伯菌的耐药监测及其?内酰胺酶研究
- Author:
Yinmei YANG
;
Zhaohui LIU
;
Jinlong CHEN
;
Huifen YE
;
Huiling CHEN
;
Yingwei YANG
- Publication Type:Journal Article
- Keywords:
Klebsiella pneumoniae;
Drug-resistance;
Isoelectric focusing
- From:
Chinese Journal of Postgraduates of Medicine
2006;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the resistance and ?-lactamase of cefoxitin-resistant Klebsiella pneumoniae. Methods The minimal inhibitory concentrations were determined by standard agar dilution.Isoelectric focusing was used to measure the PI(s) of an isolate,s ?-lactamase,AmpC and ESBLs activity was confirmed by a three-dimensional extract method. Results The resistant rates of 40 strains were as follows: imipenem and meropenem 0.0%,cefepime 20.6%,cefotaxime(22.5%),ceftazidime 60.0%.The most isolates were demonstrated two or more ?-lactamase bands by IEF.Of 39 strains tested,ESBLs was detected in 31(70.5%) strains and AmpC-type?-lactamase in 16(41.0%) strains by three dimensional extract test. Conclusions These cefoxitin-resistant Klebsiella pneumoniae produced two or more ?-lactamases.It is imperative for clinical microbiology laboratories to detect and research ?-lactamases,especially AmpC enzyme.
