Effect of all-trans retinoic acid on expression of gap junction genes of hepatocellular carcinoma cell lines
- VernacularTitle:全反式维甲酸对肝癌细胞株细胞连接蛋白基因表达的影响
- Author:
Bin XU
;
Jianwei WANG
;
Heqing FANG
;
Yingbin LIU
;
Yiping MU
;
Xiujun CAI
;
Jiangtao LI
;
Shuyou PENG
- Publication Type:Journal Article
- Keywords:
Carcinoma, hepatocellular;
Gene expression;
All-trans retinoic acid;
Connexin gene
- From:
Chinese Journal of General Surgery
1993;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of all-trans retinoic acid (ATRA) on expression of gap junction genes 26, 32 and 43 in two human hepatocellular carcinoma cell lines. Method Human hepatocellular carcinoma cell lines SMMC-7721 and BEL-7404 were cultured and inoculated in culture plate. Cells were divided into 2 groups and cultured respectively with normal medium and medium contains ATRA at a concentration of 10-mol/L. After 24, 48 and 72 hours, cells were collected and total mRNA were extracted. RT-PCR was used to detect the transcription of gap junction genes 26, 32 and 43. Result SMMC-7721 and BEL-7404 cultured under condition of normal medium do not express CX26 mRNA and CX32 mRNA. With the effect of ATRA for 48 and 72 hours, SMMC-7721 cell expressed CX26 mRNA and CX32 mRNA. BEL-7404 expressed CX26 mRNA and CX32 mRNA while cultured with ATRA for 72 hours. CX43 mRNA expression of the two cell lines was not altered by ATRA. Conclusion ATRA affects the expression of CX26 and CX32 in HCC cell lines SMMC-7721 and BEL-7404 at the transcription level.
