The effect of IGF- I receptor monoclonal antibody on human colorectal cancer cell lines
- VernacularTitle:胰岛素样生长因子Ⅰ型受体单克隆抗体对结肠癌细胞生长的影响
- Author:
Yawu ZHANG
;
Youcheng ZHANG
;
Xiaoming ZHANG
;
Zhimin KOU
;
Xusheng LI
- Publication Type:Journal Article
- Keywords:
Colonic neoplasms;
Receptor, IGF type 1;
Antibodies, monoclonal
- From:
Chinese Journal of General Surgery
1993;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the expression of insulin-like growth factors and their receptor (IGF- I R) in a human colorectal cancer cell line HT-29, and the effect of two IGF- I R monoclonal antibodies on the biological behavior of HT-29 cell lines. Methods Immunohistochemistry was used to test the expression of IGF- I R in HT-29 cell lines. MTT assay was used to determine the anti-proliferation effects of IGF- I R monoclonal antibody (McAb). Apoptosis percentage was estimated by flow cytometry ( FCM). Results IGF- I R was expressed in the membranes of HT-29 cell lines. McAb of IGH- I R can inhibit the proliferation of HT-29 cell lines in a dose-dependent manner. The apoptosis rate of HT-29 cells treated by IGF- I R McAb was higher than that of control group (P
