The role of NF-?B signal pathway in a rat acute colitis model
- VernacularTitle:NF-?B信号传导通路在大鼠急性结肠炎应激反应中的作用
- Author:
Jing ZHOU
;
Yi FENG
;
Shan WANG
;
Yingjiang YE
- Publication Type:Journal Article
- Keywords:
NF-kappa B;
Colitis;
Stress;
Animal experimentation
- From:
Chinese Journal of General Surgery
1993;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of NF-?B signal pathway in the stress of a rat acute colitis model. Methods Acute colitis were induced by 6% intracolonic acetic acid lavation in rats; the spinal I-?B levels were measured by Western blot at L5-S1 and C2-4 levels; the spinal COX-2 mRNA levels were measured by RT-PCR at the same segments; the expressions of spinal COX-2 were also measured by immunohistochemistry. Results There were obvious regional inflammatory responses after intracolonic acetic acid lavation in rats; the extent of inflammation was gradually aggravated with time.The expressions of spinal I-?B at L5-S1 level significantly increased after 0. 5 h in acute colitis group. Expressions of experimental group were significantly higher than that of sham group at half an hour(P
