Effect of remifentanil on electrically induced intracellular Ca~(2+) transients in rat ventricular cardiomyocytes
- VernacularTitle:瑞芬太尼对电刺激诱导大鼠心室肌细胞钙瞬变的影响
- Author:
Ye ZHANG
;
Erwei GU
;
Jian ZHANG
- Publication Type:Journal Article
- Keywords:
Piperidines;
Heart ventricles;
Myocardium;
Calcium
- From:
Chinese Journal of Anesthesiology
1994;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To elucidate the direct effect of remifentanil on intracellular Ca2+ ( [Ca2+ ]) transients using electrically stimulated individual rat ventricular cardiomyocytes. Methods Male SD rats weighing 190-210 g were used. The animals were decapitated and their hearts were immediately removed. Fresh ventricular cardiomyocytes were enzymatically isolated with collagenase. For measurement of the electrically induced [ Ca2 + ] transients, cells were first loaded with fura-2/AM as a Ca2+ indicator and [Ca2+ ] transient was determined by spectrofluorometric method. The fluorescence ratio of 340/380 run was used as an index of [ Ca2+]. Myocytes were placed in a chamber and perfused with a bicarbonate Kreb's solution at room temperature. Myocytes were randomly divided into 10 groups according to the different concentrations of remifentanil contained in Kreb's solution: control group (no remifentanil n = 8) and 9 remifentanil groups (0.1, 0.3, 1.0, 3.0, 10, 30, 100, 300 and 1 000 ng ?ml-1) ( n = 8 each) .Results Remifentanil caused decrease in [Ca2+ ] transients in a dose-dependent manner. Except for group remifentanil 0.1 and remifentanil 0.3, [ Ca2+] transients were significantly lower in remifentanil groups than in control group (P
