Protective effect of isoflurane preconditioning on the brain against ischemia-reperftision injury in gerbils
- VernacularTitle:异氟醚预处理对沙士鼠脑缺血再灌注损伤的保护作用
- Author:
Jie SHEN
;
Zhen SUN
;
Haijun LIU
- Publication Type:Journal Article
- Keywords:
Isoflurane;
Reperfusion injury;
Brain
- From:
Chinese Journal of Anesthesiology
1994;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effect of isoflurane preconditioning on the brain against ischemia-reperfusion (I/R) injury and the underlying mechanism.Methods Seventy-five male gerbils weighing 60-80 g were randomly divided into 5 groups ( n = 15 each): group A sham operation (sham) ; group B I/R; group C isoflurane + I/R (ISO); group D 5-HD (mito-KATP channel blocker) + I/R (5-HD) and group E 5-HD + isoflurane + I/R (5-HD+ISO). The animals were anesthetized with intraperitoneal (i.p.) 10% chloral hydrate 0.35 g?kg-1 . Global cerebral I/R was produced by occlusion of bilateral common carotid arteries for 5 min and confirmed by isoelectric potential on EEG. In ISO group the animals inhaled 1.2%-1.5% isoflurane for 30 min followed by 30 min wash-out period before I/R. In 5-HD group 5-HD 10 mg?kg-1 was injected i.p. 30 min before I/R. In 5-HD+ ISO group 30 min after i.p. 5-HD 10 mg?kg-1 , isoflurane was inhaled as in ISO group. The animals were killed 24 h after global cerebral ischemia and their forebrains were excised for (1) electron microscopic examination of mitochondria and (2) measurement of mitochondrial permeability transition pore (MPTP) (ultraviolet spectrophotometer) and (3) calcium content in mitochondria (spectrophoto-fluorometer) . Results Marked swelling of mitochondria with disrupted cristae and damaged matrix were observed in group I/R (B) , 5-HD (D) and 5-HD + ISO (E) while in sham (A) and ISO (C) groups relative intact mitochondria were seen. The mitochondrial Ca2+ concentration was significantly higher and MPTP more open in I/R (B), 5-HD (D) and 5-HD+ ISO (E) groups than in sham (A) and ISO (C) groups (P
