Hyperthermia: its effectiveness in killing tumor cells and influence on Na~+ -K~+ -ATPase activities in erythrocytes
- VernacularTitle:热处理对回收血中肿瘤株细胞的杀伤作用和对红细胞Na~+-K~+-ATP酶活性的影响
- Author:
Jianbo WU
;
Weifu LEI
;
Liyun ZHAO
- Publication Type:Journal Article
- Keywords:
Hyperthermia, induced;
Tumor cells, cultured;
Erythrocyters;
Na( + )-K( + ) exchanging ATPase
- From:
Chinese Journal of Anesthesiology
1994;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effectiveness of hyperthermia of different degrees in killing tumor cells and its influence on Na+ -K+ -ATPase activities in erythrocytes. Methods Cultured low differentiated stomach gland tumor cells (SGC-170) and large intestine gland tumor cells (LOVO) (1?106?ml-1 each) were mixed with concentrated RBCs respectively and incubated in warm bath of 37℃ , 42℃, 43℃ , 45℃ or 47℃ for 40 min respectively. After hyperthermic treatment tumor cells were isolated from RBCs using density gradient centrifugation and the live tumor cells were counted by typan staining. The isolated tumor cells were then cultured and the clone formation of tumor cells was checked. The cultured tumor cells were marked with 5-bromo deoxyuridine and DNA metabolism was examined. The Na+ -K+ -ATPase activities in RBC after hyperthermic treatment were also determined. Results The amount of tumor cells was significantly decreased by 40 min hyperthermic treatment in a temperature-dependent manner from 42-471 as compared with the control group (37℃) (P
