Assaying of Fluoxetine in Microdialysate in Rat's Brain by HPLC
- VernacularTitle:高效液相色谱法测定大鼠脑微渗析液中氟西汀的浓度
- Author:
Yingchun LI
- Publication Type:Journal Article
- Keywords:
Fluoxetine;
HPLC;
Microdialysis
- From:
China Pharmacy
1991;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To establish a HPLC method for the assaying of fluoxetine in microdialysate in rat's brain by HPLC. METHODS: Fluoxetine, which was extracted with N-hexane, was separated on ODS chromatographic column after derivatization by NBD-COCl,acetonitrile-water (50∶50) was the mobile phase with a flow rate of 1.2ml/min, column temperature of 35℃,fluorescence detection wave length ?ex at 478nm and ?em at 537nm. RESULTS: The linear range for fluoxetine in microdialysate in rats' brain was 10～100 nmol/L(r=0.9 996), and the quantitation limit was 10 nmol/L. CONCLUSIONS: The method is specific, sensitive and accurate,it is suitable for the assaying of fluoxetine in biological specimen.
