Effects of myofibrillogenesis regulator on myocardial hypertrophy
- VernacularTitle:肌原纤维调节因子-1在心肌肥大中的作用研究
- Author:
Feifei XU
;
Xiuhua LIU
;
Yanzhen WANG
;
Tianbo LI
;
Yiguang WANG
- Publication Type:Journal Article
- Keywords:
Myofibrillogenesis regulatory factor;
Myocardium;
Hypertrophy;
Angiotensin Ⅱ
- From:
Chinese Journal of Pathophysiology
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effects of myofibrillogenesis regulator-1 (MR1) on myocardial hypertrophy. METHODS: Three stem-loop structures of rMR1 mRNA were selected as targets to establish RNA interference carriers. After transient transfection with plasmids, cultured cardiomyocytes of neonatal were used to perform RT-PCR for choosing the first target to carry out RNA interference blocking MR1 gene. In order to observe the effect of MR1 gene silence on myocardial hypertrophy induced by angiotensin Ⅱ (AngⅡ), the radiation intensity of tritium-leucine ([3H]-Leu) was used to label the cardiomyocytes. Morphological observation, protein extraction and Western blotting were also used to investigate protein synthesis rate, cell surface area and expression of rMR1. RESULTS: The radiation intensity of tritium-Leucine in AngⅡ group increased 21.4% (P
