Changes of the myocardium energy metabolism and adenine nucleotide translocase activity in rats after hypoxic exposure
- VernacularTitle:缺氧对大鼠心肌线粒体能量代谢和腺苷酸转位酶活性的影响
- Author:
Bing LI
;
Junze LIU
;
Lifen CHEN
- Publication Type:Journal Article
- Keywords:
Anoxia;
Mitochondria;
Energy metabolism;
Adenine nucleotide translocase;
Myocardium
- From:
Chinese Journal of Pathophysiology
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To explore the changes of myocardial energy metabolism and adenine nucleotide translocase (ANT) activity in mitochondria in rats exposed to hypoxia. METHODS: Adult male Wistar rats were exposed to simulated high altitude at 5 000 m for control (0 d), 1 d, 5 d, 15 d, 30 d in hypobaric chamber. Myocardial mitochondria were isolated by centrifugation. Mitochondria respiratory function was measured by Clark oxygen electrode. The size of adenine nucleotides pool (ATP, ADP, AMP) in mitochondria were separated and measured by HPLC. ANT activity was measured by [3H]-ADP incorporation. RESULTS: Compared to control, mitochondria state Ⅲ respiratory (ST_3) and RCR decreased and ST_4 increased sharply at 1 d, 5 d and 15 d, ST_3 still lower than that in control at 30 d, while RCR level restored. ATP contents and ANT activity decreased at 1 d and 5 d, then restored to control level at 15 d, then decreased again at 30 d. CONCLUSION: The inhibition of mitochondria respiratory function is the main reason that makes ATP contents decrease during hypoxic exposure. ANT activity and ATP content change cooperatively.
