Quantitive analysis of mitochondrial DNA deletion in mice with viral myocarditis
- VernacularTitle:病毒性心肌炎小鼠心肌细胞线粒体DNA缺失的定量分析
- Author:
Jin WEI
;
Dengfeng GAO
;
Xiaolin NIU
;
Jian LIU
- Publication Type:Journal Article
- Keywords:
Myocarditis;
Coxsackievirus infections;
Mice;
DNA, mitochondrial
- From:
Chinese Journal of Pathophysiology
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To elucidate the role of mitochondrial DNA (mtDNA) deletion in the pathogenesis of viral myocarditis in mice. METHODS: 50 BALB/c mice were divided into two groups randomly. 40 were experimental group, each of them was injected 0.1 mL Eagle liquids with CVB_3 (TCID_ 50=108) intraperitoneally. Another 10 mice were given equal volume Eagle liquids as control group. Cardiac functions in vivo and mtDNA 3867 deletion rate in myocytes were detected separately at the day 3, 11 and 24 after injection. The correlation of mtDNA 3867 deletion rate to cardiac functions was analyzed using Spearman method. RESULTS: At the day 3 after injection, mtDNA 3867 deletion rate in experimental group was 8.3 times higher than that in control group [(0.01970?0.00118)% vs (0.00211?0.00032)%,P
