Modulation of bacterial redox protein azurin induces human osteosarcoma cell apoptosis by Fas antigen and caspase-8
- VernacularTitle:Fas抗原和caspase-8调控细菌氧化还原蛋白azurin诱导的人骨肉瘤细胞凋亡的实验研究
- Author:
Xudong MIAO
;
Disheng YANG
;
Zhaoming YE
;
Rongzhen XU
;
Jie FENG
;
Xin HUANG
- Publication Type:Journal Article
- Keywords:
Azurin;
Osteosarcoma;
Apoptosis;
Antigen, Fas;
Caspase 8
- From:
Chinese Journal of Pathophysiology
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To determine the role of Fas antigen and caspase-8 in modulating apoptosis of osteosarcoma cells induced by bacterial redox protein azurin. METHODS: The human osteosarcoma cell line U2OS was treated with bacterial redox protein azurin (150 mg/L) for 6, 12, 24 and 48 h, respectively. Cell immunohistochemistry and quantitative image pattern analysis were applied for detecting the expression of Fas antigen. Caspase-8 activity was detected using caspase-8 fluorescent assay kit. The apoptotic rate was measured by FCM. RESULTS: Compared with the control group, the expression of Fas antigen and activity of caspase-8 significantly increased in U2OS cells treated with 150 mg/L azurin (P
