Ischemic preconditioning relieves the ischemia/reperfusion injury of neurons in hippocampus by inhibiting the expression of p53
- VernacularTitle:缺血预处理经抑制p53表达减轻缺血再灌后大鼠海马神经元损伤
- Author:
Huimin LIU
;
He CUI
;
Yajun LIU
;
Fuyu XU
;
Zhihong WANG
;
Lianbi CHEN
- Publication Type:Journal Article
- Keywords:
Rats;
Hippocampus;
Ischemic preconditioning;
Genes, p53;
Gene expression
- From:
Chinese Journal of Pathophysiology
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To examine whether ischemic preconditioning (IPC) can protect against apoptosis in CA1 subfield of hippocampus following reperfusion of a lethal ischemia in rats and explore the role of IPC by inhibiting the expression of p53 in this process. METHODS: Wistar rats were used in the experiment. A global ischemia/reperfusion model was induced by 4-vessel occlusion. The rats were divided into the following three groups randomly: (1) ischemic preconditioning group (IPC group); (2) ischemia/reperfusion group (IR group); (3) control group. The histopathological changes, the percentage of apoptosis and the expression of p53 gene in CA1 region of rat hippocampus were examined by HE staining, FCM, RT-PCR and immunohistochemistry techniques. RESULTS: The neuronal density of CA1 region in IPC group [(217?9)/0.72 mm2] was significantly higher than that in IR group [(29?5)/0.72 mm2, P
