Effects of sterigmatocystin on IL-4 mRNA expression and protein secretion of murine spleen cells in vitro
- VernacularTitle:杂色曲霉素对小鼠脾细胞IL-4mRNA表达和蛋白分泌的影响
- Author:
Lingxiao XING
;
Xianghong ZHANG
;
Guiran YIN
;
Yuehong LI
;
Junling WANG
;
Xia YAN
;
Fengrong WANG
- Publication Type:Journal Article
- Keywords:
Sterigmatocystin;
Spleen;
Interleukin-4;
mRNA expression
- From:
Chinese Journal of Pathophysiology
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To explore the effects of sterigmatocystin (ST) on IL-4 expression and secretion of murine spleen cells in vitro. METHODS: The secretion and expression of IL-4 in murine spleen cells were studied with ELISA and semi-quantitative RT-PCR method after ST pretreatment at five different dosages (0.125 mg/L, 0.25 mg/L, 0.5 mg/L, 1 mg/L, 2 mg/L) for 2 h and 12 h, respectively. RESULTS: Semi-quantitative RT-PCR analysis showed that the expression of IL-4 mRNA in ST 0.125, 0.25 and 0.5 mg/L treated groups were higher than that in control group, especially in ST 0.5 mg/L group ( P
