Regulatory effects of Kv on the tension of normal and passively sensitized human airway smooth muscle
- VernacularTitle:Kv在正常与被动致敏人气道平滑肌张力调控中的作用
- Author:
Dongjun CHENG
;
Yongjian XU
;
Xiansheng LIU
;
Limin ZHAO
;
Shengdao XIONG
;
Zhenxiang ZHANG
- Publication Type:Journal Article
- Keywords:
Potassium channels;
Airway;
Muscle, smooth;
Asthma
- From:
Chinese Journal of Pathophysiology
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the regulatory effects of voltage-dependent delayed rectifier potassium channel (Kv) on the tension of normal and passively sensitized human airway smooth muscle (HASM). METHODS: By using blockers of potassium channels as tools, the tension of HASM and Kv gene mRNA and protein expressions in normal and asthmatics serum sensitized HASM cells were measured with techniques of reverse transcriptase/polymerase chain reaction (RT-PCR) and immunocytochemistry. RESULTS: (1) 4-aminopyridine (4-AP), the blocker of Kv, caused a concentration dependent constriction in normal HASM rings. The negative logarithm of the drug concentration causing 50% of maximal effect (pD_2) in normal group (2.09?0.09) was significantly different from that in the sensitized group (2.44?0.16, P0.05). (2) There were Kv1.2, Kv1.3 and Kv1.5 mRNA expressions in cultured HASM cells, but only Kv1.5 mRNA (P
