Ribozyme mediated inhibition on GBC-SD gallbladder carcinoma cells
- VernacularTitle:核酶切割技术对胆囊癌细胞端粒酶活性抑制作用的研究
- Author:
Bin JIN
;
Wei WANG
;
Xihong JIANG
;
Sanyuan HU
;
Chaoyang ZHANG
;
Xiaoling QIAO
- Publication Type:Journal Article
- Keywords:
Gallbladder Carcimoma Cell Line;
Ribozyme Mediated Inhiliting;
Gene Therapy
- From:
Chinese Journal of General Surgery
1993;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of ribozyme mediated inhibition on telomerase activity and cell(proliferation) of GBC-SD cells.Methods According to the hTR template two wings region sequence,the hammerhead ribozyme gene sequence was synthesized in vitro.Then,it was engineered into the eukaryon(expression) vector pTriEx-4.Lipofectamine was used to transfect the carcinoma of gallbladder.The inhibition of telomerase activity and cell proliferation in GBC-SD cell was observed,and compared with control group.(Results) Compared to control group,ribozyme can significantly inhibit telomerase activity and cell proliferation of GBC-SD cell.After ribozyme action,the cell ratio of G_0/G_1 markedly increased,and the cell ratio of S phase markedly decreased.Cellular cleavage and proliferation was markedly inhibited.The apoptosis rate was 17.10% and 31.01% on day10 and day13 after ribozyme action.Conclusions Ribozyme can inhibit(telomerase) activity of GBC-SD cells effectively and induce cell apoptosis.
