Immune responses and anti-tumor effects of melanoma antigen-n protein vaccine accompanied by CpG-containing oligodeoxynucleotide adjuvant
- VernacularTitle:以CpG-ODN为佐剂的MAGE-n蛋白疫苗的免疫学作用及抗肿瘤效应
- Author:
Yayu HUANG
;
Wei TONG
;
Jiahai MA
;
Jing YE
;
Guangsheng CHEN
;
Yanfang SUI
- Publication Type:Journal Article
- Keywords:
Melanoma;
Antigens,neoplasm;
Vaccines;
CpG islands;
Oligodeoxyribonucleotides
- From:
Journal of Chinese Physician
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To prepare melanoma antigen n(MAGEn)protein vaccine and to investigate the immune responses and anti-tumor effects of MAGE-n protein vaccine accompanied by CpG-containing oligodeoxynucleotide(CpG-ODN)adjuvant.Methods The DH5? containing the MAGE-n prokaryotic expression plasmid pGEX-MAGE-n was induced and the protein was purified as protein vaccine.The CpG-ODN was synthesized as adjuvant and the C57BL/6 mice were inoculated.The cellular and humoral immune responses were detected by ELISPOT,cytotoxicity assay and enzyme linked immunosorbent assay(ELISA).The antitumor effects were detected through tumor volume and life span.Results The MAGE-n protein accompanied by CpG-ODN could induce strong MAGE-n-specific cellular and humoral immune responses.In the MAGE-n positive B16 tumor model of C57BL/6,the growth velocity of tumor was decreased and the life span was prolonged with the treatment of vaccine.Conclusion MAGE-n protein vaccine accompanied by CpG-ODN adjuvant can induce strong immune responses and anti-tumor effects against MAGE-n positive B16 tumor,which provides a new way for tumor therapy.
